Development of two diagnostic tools (ELISA and ICT) for detection of antibodies against ovine and bovine theileriosis
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Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene
Background: The use of IgYs in a variety of methods in different areas of research, diagnostics, medical application and biotechnology should be considered widely. Objectives: Development of antibodies against extra cellular domain of influenza M2 (M2e) protein in egg yolk of laying hens. Methods: A Fusion construct harboring C-terminal of bovine heat shock protein 70 (Hsp70) and influenza M2e ...
متن کاملProduction of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)
Bovine Viral Diarrhea virus (BVDV) is an important viral pathogen of cattle causing several clinical syndromes. There are usually no pathognomonic clinical signs of BVDV infection. Diagnostic investigations therefore rely on serological detection and virus isolation. Nonstructural protein 3 (NS3) as immunogenic protein of BVDV is genetically and antigenically conserved among different isolates....
متن کاملDevelopment of a Western Blot Assay for Detection of Antibodies against HSV Using Purified HSV Virions Prepared by Sucrose Density Gradient
Objective(s) Herpes simplex viruses (HSVs) have widespread and ubiquitous prevalence in the human population and they have received a great deal of attention due to the range of diseases, they caused as a result of an infection. It seems that the fast and reliable diagnostic methods are needed for detecting the herpes simplex virus type 1 (HSV1) antibodies especially in patients with HSV encep...
متن کاملProduction of Monoclonal Antibody against Prokaryotically Expressed G1 Protein of Bovine Ephemeral Fever Virus
Epitope-G1 of bovine ephemeral fever virus (BEFV) G glycoprotein has been genetically and antigenically conserved among various isolates of BEFV and only reacts with anti-BEFV neutralising antibodies. Therefore, it is a candidate antigen for development of the enzyme linked immunosorbent assay (ELISA) for serological identification bovine ephemeral fever (BEF)-infected animals. The aim of this ...
متن کاملAdaptation of an indirect enzyme-linked immunosorbent assay by purified gp51SU for detection of antibodies to bovine leukemia virus
The objective of this study was to compare an indirect ELISA, based on a purified 60 kDa envelope glycoprotein (gp51SU), with a Pourquire indirect ELISA for the detection of antibodies to the bovine leukemia virus. For conducting this research, 340 serum samples were collected from two different breeds of cows (Sarabi and Holestin) in different herds. Commercial ELISA revealed positive results ...
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تاریخ انتشار 2011